Lab Materials Purchased from Modern Biology, Inc.
Questions
- What is the purpose of this lab?
- Which two major molecular tools have been used to create the recombined plasmid?
- What were the two sources of DNA used to create the recombined plasmid?
- You will set up four microtubes, each with different contents. What will you place in microtube 1? 2? 3? 4? Why?
- After preparing the four samples, you’ll place the microtubes in a 37 degrees Celsius water bath for 60 minutes. What is the purpose of incubating the samples? Why use a 37 degrees Celsius water bath?
- Study the restriction maps on page 5 of the lab. Predict the results of lanes 1 and 2 on a gel. In other words, how many bands do you expect to observe in lanes 1 and 2? What is the size of each fragment?
- After analyzing the results of the gel, approximately how many base pairs were inserted into the pUC 18 plasmid? How do you know?
- How many BamHI restriction sites are contained within the viral insert? How do you know?