Analysis of a Mutant Hemoglobin Gene Lab

Lab Materials Purchased from Modern Biology, Inc.

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Pre-Lab Questions

  1. Study information about DNA mutations at http://learn.genetics.utah.edu/archive/sloozeworm/mutationbg.html
  2. Study Figure 1 on page 2 of your lab handout.  What are the three types of mutations we’ll study during this lab? 
  3. What is meant by the term “wild-type”?
  4. Study the restriction maps on page 4 of the lab.  Which two restriction enzymes will be used during this lab?
  5. Which gene will be studied during this lab?
  6. After studying the lab protocol, how many fragments do you expect to see in lane 1?  Lane 3?

Analysis

  1. Study the results of the gel.  mutant-hemoglobin-gel.JPG      
  2. What type of mutation has occurred?  How do you know?
  3. Identify the rabbit DNA and plasmid DNA in lanes 3 and 4.

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