Analysis of a Mutant Hemoglobin Gene Lab
Lab Materials Purchased from Modern Biology, Inc.
Pre-Lab Questions
- Study information about DNA mutations at http://learn.genetics.utah.edu/archive/sloozeworm/mutationbg.html
- Study Figure 1 on page 2 of your lab handout. What are the three types of mutations we’ll study during this lab?
- What is meant by the term “wild-type”?
- Study the restriction maps on page 4 of the lab. Which two restriction enzymes will be used during this lab?
- Which gene will be studied during this lab?
- After studying the lab protocol, how many fragments do you expect to see in lane 1? Lane 3?
Analysis
- Study the results of the gel.
- What type of mutation has occurred? How do you know?
- Identify the rabbit DNA and plasmid DNA in lanes 3 and 4.
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