Electrophoretic Separation of Proteins Lab

Lab Materials purchased from Modern Biology, Inc.

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Questions

  1. Define the term “isoelectric point.”
  2. Study the following gel:

electrophoretic-sep-of-proteins-gel.jpg

 

Why are the wells in the middle of the gel?

The pH of the buffer in the chamber is 8.6.  Let’s assume that the red protein is protein #1 and the blue protein is protein #4.  What is the charge of each protein?  Which protein has the highest isoelectric point?  Which protein has the lowest isoelectric point?

 

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