Electrophoretic Separation of Proteins Lab
Lab Materials purchased from Modern Biology, Inc.
Questions
- Define the term “isoelectric point.”
- Study the following gel:
Why are the wells in the middle of the gel?
The pH of the buffer in the chamber is 8.6. Let’s assume that the red protein is protein #1 and the blue protein is protein #4. What is the charge of each protein? Which protein has the highest isoelectric point? Which protein has the lowest isoelectric point?
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