Micropipetting & Equipment Labs

During biotech this year, you will use certain equipment many times so it’s important to become familiar with each piece of equipment, it’s function, and proper care of it.

1.   What are the names of the three pieces of agarose gel casting equipment depicted in this picture?  What is the purpose of each? gel caster

2.  How many milliliters of hot agarose are used to pour a gel?

3.  What is the name of this instrument?  For what purpose is it used?  How is it used properly?                                                                                                   micropipettor

4.  This is a 0.5-10 µL micropipettor.  What volume is displayed in the window?

05 to 10 micropipetor

5.  Study the 2-20 µL micropipettor in the picture below.  What volume is displayed in the window?

2 to 20 micropipetor

6.  What volume is depicted on the 100-1000 µL micropipettor below?

100 to 1000 micropipetor

7.  What is the name of this piece of equipment and what is its purpose?

vortex

8.  What is the name of this piece of equipment and what is its purpose?

microcentrifuge

9.  Study the orientation of the microtubes.  Why is their placement significant?                                            balanced microcentrifuge

10.  Name this piece of equipment and state its purpose.

horizontal gel chamber

11.  Where is chamber buffer located in our lab?  What is the pupose of the chamber buffer?  Approximately how much chamber buffer is placed in a chamber that contains a gel?

chamber buffer

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