Exploring Protein Denaturation

Lab Kit Purchased from g-biosciences.jpg

Pre-Lab Questions

  1. What is the name of the protein used in this lab?  How was its name derived?
  2. Is the protein used in this lab able to renature without the help of enzymes?
  3. Will you use a vertical or horizontal gel in this lab?  Why?
  4. What is PAGE?
  5. What is SDS?

Analysis

  1. What color is the GB protein?
  2. How was the GB protein denatured?
  3. Consider the two samples of GB protein.  What does the “F” stand for?  What about “D”?
  4. Immediately after gel electrophoresis, were you able to visualize the F protein with a UV light?  What about the D protein?
  5. Why did you was the gel in water after gel electrophoresis?
  6. View the gel below.  If you look carefully, you’ll see the GB protein in the F lane.protein-folding-study-gel.JPG

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