Lab Kit Purchased from
Pre-Lab Questions
- What is the name of the protein used in this lab? How was its name derived?
- Is the protein used in this lab able to renature without the help of enzymes?
- Will you use a vertical or horizontal gel in this lab? Why?
- What is PAGE?
- What is SDS?
Analysis
- What color is the GB protein?
- How was the GB protein denatured?
- Consider the two samples of GB protein. What does the “F” stand for? What about “D”?
- Immediately after gel electrophoresis, were you able to visualize the F protein with a UV light? What about the D protein?
- Why did you was the gel in water after gel electrophoresis?
- View the gel below. If you look carefully, you’ll see the GB protein in the F lane.