Brebeuf Jesuit Biotechnology

Lab Kit Purchased from:

Learn more about Serum Protein Electrophoresis by reading the discussion of the technique here.

Questions:

1. What are the five major fractions of serum proteins?  In what order do they migrate on a gel? 
2. Assuming the electrophoresis buffer has a pH of 8.6, which group of serum proteins has the lowest isoelectric point?  How do you know?
3. Study the results of the following gel:

What was loaded in lane 1?  What samples were loaded in lanes 2-4?  What was the purpose of loading these samples in lanes 2-4? 

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