Lab Kit Purchased from

Questions to Consider:

1. What is the purpose of this lab?
2. What will happen to the dye-labeled protein over time?  Why? 
3. Notice what happens to the dye-labeled protein when it is exposed to UV light.                                            
4. What is the purpose of Proteinase K?  What type of protease is it, meaning which amino acid does it recognize?                                                                                                                     
5. What is the purpose of the stop solution?  What is the major component of the stop solution?                                         
6. What is the purpose of microtube 10 in the experiment?
7. What observable trend should you see in the intensity of the dye-labeled protein when viewing lanes 1-9?  Why?
8. What observable trend should you see in the intensity of the fragments when viewing lanes 1-9?  Why?
9. Will the dye-labeled protein or the fragments travel a greater distance in the gel?                                            

 

Application Question: Watch this short video to answer the question, “How does HIV utilize viral protease?”