Lab Materials Purchased from Modern Biology, Inc.
Pre-Lab Questions
- What is satellite DNA?
- Observe Figure 4 on page 9 of the lab handout. Why does lane A contain only one band? If the DNA in lane A and lane C were derived from the same bacterial culture, why are the banding patterns so different? Describe the results of lane D.
- What is the purpose of cutting phage lambda with a restriction enzyme in this particular lab?
Questions
- In this experiment, a 1.2% agarose gel is used. How was the gel prepared?
- How many EcoRI recognition sites does the pUC 18 plasmid contain? How many bands do you expect to see in lane 1 of the gel?
- How many HindIII recognition sites does phage lambda contain? How many bands do you expect to see in lane 2 of the gel?
- Do you expect the results in lanes 3 and 4 to be identical or different? Explain.
- What is the purpose of tracking dye?
- What is the purpose of buffer?
- What is DNA’s charge? Should you place the wells closest to the anode or cathode?
- What is the purpose of staining the gel?
- What is the purpose of destaining the gel?
- Which data will be used to construct a standard curve?
- How will you determine the size of the calf satellite DNA?
- When referring to DNA bands, what is meant by “migration distance”?
- Observe the gel below. Where is the satellite DNA located? Explain the results of lanes 3 and 4.
